Research Applications

Nitric Oxide

Nitric oxide (NO) is a short-lived small gas molecule that is found in vivo where it acts as a signalling molecule. The study of nitric oxide and its derivatives is a subject of much active research.

There are several types of detector for nitric oxide concentration that provide an analog voltage output signal compatible with e-corder. If in doubt you can ask us about specific models.

Chemiluminesence method

Chemiluminescence detectors can be use to detect nitric oxide in air or liquids. A compatible chemiluminesence detector is the:

This instrument measures nitric oxide and its reaction products, nitrite, nitrate and nitrosothiols in virtually any biological fluid. Chart software with e-corder is usually the best choice for recording the signal.

Electrochemical method

'Clark' style electrodes and a suitable electrochemical meter are most often used for measurements in solution when continuous monitoring is required. Nitric oxide is usually oxidised to nitrate at the anode of the electrode probe.

Meters and electrodes are made by:

Remember to ask if the meter has analog output signal (which is suitable for connection to
e-corder).

Most of these manufacturers' electrodes can be used with the EA162 Picostat. Using the Picostat you can adjust the polarisation of the the electrode probe (usually about +0.7 to +0.8 volts) for optimum results.

e-corder with Chart software is used to record the signal. Non-linear, multiple point, calibration of the signal is facilitated by using the MPCalibration Chart extension.

Spectroscopic method

It is also possible to determine nitric oxide concentration spectroscopically.
 
  • Eicom ENO-10 NOx analysis system

The reaction of nitric oxide with with an amine is used to produce a highly coloured molecule which is then analysed by an absorbance chromatography detector using a

This method is claimed to have greater sensitivity than the chemiluminesence method.

 


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