Anions by MCE-C4D - Revision history

Oliver: /* Equipment and Reagents Required */

2016-08-24T06:18:32Z

‎Equipment and Reagents Required

Oliver: /* Equipment and Reagents Required */

2016-04-27T07:49:01Z

‎Equipment and Reagents Required

Oliver: /* Equipment and Reagents Required */

2016-04-27T07:46:14Z

‎Equipment and Reagents Required

Oliver: /* Equipment and Reagents Required */

2016-02-22T04:22:56Z

‎Equipment and Reagents Required

Oliver: /* Equipment and Reagents Required */

2016-02-17T05:22:52Z

‎Equipment and Reagents Required

Oliver: /* Equipment and Reagents Required */

2014-10-03T02:32:17Z

‎Equipment and Reagents Required

Oliver: /* Introduction */

2014-01-07T06:25:15Z

‎Introduction

Oliver: /* Introduction */

2014-01-07T06:24:51Z

‎Introduction

Oliver at 07:57, 11 September 2013

2013-09-11T07:57:00Z

Oliver: /* Equipment and Reagents Required */

2013-09-11T07:46:07Z

‎Equipment and Reagents Required

@@ Line 9: / Line 9: @@
 == Equipment and Reagents Required ==
-* [Http://www.edaq.com/ER455 ER455] Microchip Electrophoresis bundles, including:
+* [Http://www.edaq.com/ER455 ER455] Microchip Electrophoresis kit, including:
 ** [Http://www.edaq.com/ER225 ER225 C4D Data System]
 ** [http://www.edaq.com/ET225 ET225 Micronit Platform]
 ** [http://www.edaq.com/ER430 ER430 High Voltage Sequencer]
 ** [http://www.edaq.com/ES280 PowerChrom] and [http://www.edaq.com/locked/software/sequencer.php QuadSequencer] softwares
-* [http://www.edaq.com/ET145-4 ET145-4 CE Microchip (45 mm) kit]
+** [http://www.edaq.com/ET145-4 ET145-4 CE Microchip (45 mm) kit]
 * Background Electrolyte (BGE) = 70 mM TRIS/CHES with 1ppm HDMB, where TRIS is Tris(hydroxymethyl)aminomethane, CHES is 2-(Cyclohexylamino)ethanesulfonic acid, and HDMB is hexadimethrine bromide
 * Samples = 100 μM and 10 μM Cl<sup>-</sup>, NO<sub>3</sub><sup>-</sup> and HSO<sub>4</sub><sup>-</sup> in deionised water.

@@ Line 14: / Line 14: @@
 ** [http://www.edaq.com/ER430 ER430 High Voltage Sequencer]
 ** [http://www.edaq.com/ES280 PowerChrom] and [http://www.edaq.com/locked/software/sequencer.php QuadSequencer] softwares
 * Background Electrolyte (BGE) = 70 mM TRIS/CHES with 1ppm HDMB, where TRIS is Tris(hydroxymethyl)aminomethane, CHES is 2-(Cyclohexylamino)ethanesulfonic acid, and HDMB is hexadimethrine bromide
 * Samples = 100 μM and 10 μM Cl<sup>-</sup>, NO<sub>3</sub><sup>-</sup> and HSO<sub>4</sub><sup>-</sup> in deionised water.

@@ Line 13: / Line 13: @@
 ** [http://www.edaq.com/ET225 ET225 Micronit Platform]
 ** [http://www.edaq.com/ER430 ER430 High Voltage Sequencer]
-** [http://www.edaq.com/ET145-4 ET145-4 CE Microchip (45 mm) kit]
+** [http://www.edaq.com/ES280 PowerChrom] and [http://www.edaq.com/locked/software/sequencer.php QuadSequencer] softwares
-** [http://www.edaq.com/ES280 PowerChrom] and [http://www.edaq.com/locked/software/sequencer.php Sequencer] softwares
 * Background Electrolyte (BGE) = 70 mM TRIS/CHES with 1ppm HDMB, where TRIS is Tris(hydroxymethyl)aminomethane, CHES is 2-(Cyclohexylamino)ethanesulfonic acid, and HDMB is hexadimethrine bromide
 * Samples = 100 μM and 10 μM Cl<sup>-</sup>, NO<sub>3</sub><sup>-</sup> and HSO<sub>4</sub><sup>-</sup> in deionised water.

@@ Line 9: / Line 9: @@
 == Equipment and Reagents Required ==
-* [Http://www.edaq.com/ER455K ER455K] Microchip Electrophoresis bundles, including:
+* [Http://www.edaq.com/ER455 ER455] Microchip Electrophoresis bundles, including:
 ** [Http://www.edaq.com/ER225 ER225 C4D Data System]
 ** [http://www.edaq.com/ET225 ET225 Micronit Platform]

@@ Line 9: / Line 9: @@
 == Equipment and Reagents Required ==
-* [Http://www.edaq.com/ER455 ER455] Microchip Electrophoresis bundles, including:
+* [Http://www.edaq.com/ER455K ER455K] Microchip Electrophoresis bundles, including:
 ** [Http://www.edaq.com/ER225 ER225 C4D Data System]
 ** [http://www.edaq.com/ET225 ET225 Micronit Platform]

@@ Line 7: / Line 7: @@
 == Introduction ==
 The three inorganic anions Cl<sup>-</sup>, NO<sub>3</sub><sup>-</sup> and HSO<sub>4</sub><sup>-</sup> were separated using microchip electrophoresis (MCE). These anions were then detected using the eDAQ C4D system.
 == Equipment and Reagents Required ==

@@ Line 1: / Line 1: @@
-[[File:Chip diag.jpg|300px|thumb|right|]]
+[[File:Chip diag.jpg|300px|thumb|right|Figure 1. Chip nomenclature]]
-[[File:Electropherogram of 100 µM Cl–, NO3– and HSO4–.jpg|300px|thumb|right|Figure 1. Electropherogram of 100 μM Cl–, NO3– and HSO4–.]]
+[[File:Electropherogram of 100 µM Cl–, NO3– and HSO4–.jpg|300px|thumb|right|Figure 2. Electropherogram of 100 μM Cl<sup>-</sup>, NO<sub>3</sub><sup>-</sup> and HSO<sub>4</sub><sup>-</sup>.]]
-[[File:Electropherogram of 10 µM Cl–, NO3–, HSO4–.jpg|300px|thumb|right|Figure 2. Electropherogram of 10 μM Cl–, NO3–, HSO4–.]]
+[[File:Electropherogram of 10 µM Cl–, NO3–, HSO4–.jpg|300px|thumb|right|Figure 3. Electropherogram of 10 μM Cl<sup>-</sup>, NO<sub>3</sub><sup>-</sup>, HSO<sub>4</sub><sup>-</sup>.]]
 ''The eDAQ C4D system was used to detect three inorganic anions, separated by microchip electrophoresis, by using contactless conductivity detection.''
 == Introduction ==
-The three inorganic anions Cl–, NO3– and HSO4– were separated using microchip electrophoresis (MCE). These anions were then detected using the eDAQ C4D system.
+The three inorganic anions Cl<sup>-</sup>, NO<sub>3</sub><sup>-</sup> and HSO<sub>4</sub><sup>-</sup> were separated using microchip electrophoresis (MCE). These anions were then detected using the eDAQ C4D system.
 == Equipment and Reagents Required ==
@@ Line 16: / Line 16: @@
 ** [http://www.edaq.com/ES280 PowerChrom] and [http://www.edaq.com/locked/software/sequencer.php Sequencer] softwares
 * Background Electrolyte (BGE) = 70 mM TRIS/CHES with 1ppm HDMB, where TRIS is Tris(hydroxymethyl)aminomethane, CHES is 2-(Cyclohexylamino)ethanesulfonic acid, and HDMB is hexadimethrine bromide
-* Samples = 100 μM and 10 μM Cl–, NO3– and HSO4– in deionised water.
+* Samples = 100 μM and 10 μM Cl<sup>-</sup>, NO<sub>3</sub><sup>-</sup> and HSO<sub>4</sub><sup>-</sup> in deionised water.
 * The following sequence was used with the High Volage Sequencer:
@@ Line 29: / Line 29: @@
 == Data Analysis and Limit of Detection ==
-Figure 1 shows the electropherogram of the 100 μM Cl–, NO3– and HSO4– solution. In order to measure the limit of detection of the anions, a 10 μM solution of the same analytes was analyzed. The electropherogram is shown in Figure 2.
+Figure 2 shows the electropherogram of the 100 μM Cl<sup>-</sup>, NO<sub>3</sub><sup>-</sup> and HSO<sub>4</sub><sup>-</sup> solution. In order to measure the limit of detection of the anions, a 10 μM solution of the same analytes was analyzed. The electropherogram is shown in Figure 3.
-Using the definition of limit of detection as equal to three times the standard deviation of signal (during four seconds before the first peak), the limit of detection is 3.7 μM for Cl– and NO3–, and 1.3 μM for HSO4–.
+Using the definition of limit of detection as equal to three times the standard deviation of signal (during four seconds before the first peak), the limit of detection is 3.7 μM for Cl<sup>-</sup> and NO<sub>3</sub><sup>-</sup>, and 1.3 μM for HSO<sub>4</sub><sup>-</sup>.