C4D Profiler in PowerChrom Software - Revision history

Oliver at 03:42, 12 September 2017

2017-09-12T03:42:59Z

2017-09-12T03:42:16Z

2014-01-07T06:53:05Z

‎Procedure

2013-11-26T06:48:20Z

‎Procedure

2013-11-25T05:13:34Z

2013-07-10T11:59:48Z

‎Equipment Required

2013-07-10T11:57:27Z

‎Equipment Required

2013-07-10T07:33:20Z

2013-07-10T07:32:39Z

2013-07-08T11:36:40Z

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 {{#ev:youtube|bh3EfUjgGGU|300|right|Video for C4D Profiler in PowerChrom Software}}
 '''This application note is obsolete. You should use the C4D Profiler V2 software to optimize C4D settings, not the C4D Profiler in PowerChrom software. See the new application note [https://www.edaq.com/wiki/Optimizing_the_C4D_Settings_using_the_C4D_Profiler_V2_Software here].'''
-== Introduction ==
 This application note describes the C4D Profiler in the PowerChrom software. The software tool is designed to help select the optimum operating parameters for your buffer/background electrolyte. The software automatically records the detector’s response at every combination of frequency, amplitude and headstage gain setting, and displays the data as a series of plots. This tool also provides a useful diagnostic tool in case of problems.

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 {{#ev:youtube|bh3EfUjgGGU|300|right|Video for C4D Profiler in PowerChrom Software}}
-''The C4D Profiler in the PowerChrom software can be used to find the best C4D settings for your background electrolyte.''
 == Introduction ==
 This application note describes the C4D Profiler in the PowerChrom software. The software tool is designed to help select the optimum operating parameters for your buffer/background electrolyte. The software automatically records the detector’s response at every combination of frequency, amplitude and headstage gain setting, and displays the data as a series of plots. This tool also provides a useful diagnostic tool in case of problems.

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 == Procedure ==
 # Turn on the C4D unit and connect to the computer.
-# Inject the background electrolyte you intend to use for experiments into the capillary. Ensure the outside of the capillary is dry by wiping with a cloth. Feed the capillary into the headstage. If you are doing microchip electrophoresis, inject the background electrolyte into the chip’s channel and place the chip on the ET121 Platform.
+# Inject the background electrolyte you intend to use for experiments into the capillary. Ensure the outside of the capillary is dry by wiping with a cloth. Feed the capillary into the headstage. If you are doing microchip electrophoresis, inject the background electrolyte into the chip’s channel and place the chip on the ET225 Platform.
 # Don’t perform this test with no solution in the capillary as this won’t give a signal.
 # Open the PowerChrom software.

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 #* If you are using a background electrolyte with high conductivity, all the blue plots may go above 2V and overload the detector. In this case, you should only use HG Off.
 # Perform a scan using the correct headstage gain setting.
-# Dilute the background electrolyte by approximately 5% using deionized water, and inject it into the capillary.
+# Dilute the background electrolyte by approximately 5% using deionized water (95% background electrolyte and 5% deionized water) and inject it into the capillary.
 # Perform a scan of the diluted background electrolyte.
 # Nominate the second (diluted) scan as a background file by clicking “Set Bg”. This allows the run to be used as a background run for subsequent operations.

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 # Performing the above scan provides a good first order indication of a reasonable choice for an operating point, but there is a potentially a better method available as described below.
 # First identify if headstage gain needs to be on (HG On) or off (HG On):
-# If you are using a background electrolyte with low conductivity, the response for all the plots may be under 2 V. In this case, you should only use HG On.
+#* If you are using a background electrolyte with low conductivity, the response for all the plots may be under 2 V. In this case, you should only use HG On.
-# If you are using a background electrolyte with high conductivity, all the blue plots may go above 2V and overload the detector. In this case, you should only use HG Off.
+#* If you are using a background electrolyte with high conductivity, all the blue plots may go above 2V and overload the detector. In this case, you should only use HG Off.
 # Perform a scan using the correct headstage gain setting.
 # Dilute the background electrolyte by approximately 5% using deionized water, and inject it into the capillary.

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 * [Http://www.edaq.com/ER225 ER225 C4D Data System]
 * The latest version of [http://www.edaq.com/ES280 PowerChrom software]
-* A C4D headstage or platform, such as the [http://www.edaq.com/ET120 ET120 C4D Headstage for Capillary Electrophoresis] or [http://www.edaq.com/ET225 ET225  Micronit Chip Electrophoresis Platform]
+* A C4D headstage or platform, such as the [http://www.edaq.com/ET120 ET120 C4D Headstage] or [http://www.edaq.com/ET225 ET225 Micronit Platform]
 * A short length of capillary, tubing or a microfluidic chip, depending on your experiment.
 * The background electrolyte you intend to use for analysis.

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 # The recording range should be set to 50 mV (to record the small changes in conductivity which your analytes will produce) and you should click Zero before analyzing your samples.
 == Identifying the Optimum C4D Settings ==
 Figure 3 shows the background subtracted plots for a low conducting background electrolyte. It shows which C4D settings produced the highest output for the small change in conductivity, approximately 90mV at 100% amplitude, 1200 kHz frequency and headstage gain on.