Procedure for Capillary Electrophoresis with C4D - Revision history

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 # Connect the computer to the C4D hardware, following the instructions in the [https://www.edaq.com/edaq-product-manuals manuals].
 # Connect the C4D hardware unit to the third-party CE instrument: connect the trigger cable and/or signal cable, depending on whether you are using eDAQ or third-party software. Follow the instructions in the relevant [https://www.edaq.com/wiki/Application_Notes#C4D_Conductivity_Detector Application Note]
-# Connect the C4D headstage to the capillary in the instrument.
+# Connect the C4D headstage to the capillary in the instrument. Ensure that you position the C4D headstage near the end of the capillary that is grounded, away from the end of the capillary where the high voltage is applied. This is to ensure that the high voltage doesn’t arc from the background electrolyte inside the capillary, through the thin wall of the capillary, to the headstage and on to the C4D hardware, which could damage the equipment.
 # Turn on the C4D hardware.
 # Open the PowerChrom software.

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 # Set the required values for Excitation Frequency and Amplitude, and set the Headstage Gain on or off. The optimum settings can be determined using [https://www.edaq.com/ES770 C4D Profiler V2 Software]. This is described in a [https://www.edaq.com/wiki/Optimizing_the_C4D_Settings_using_the_C4D_Profiler_V2_Software separate application note].
 # Inject your background electrolyte (BGE) into the capillary. This can done using the instrument’s controls. Alternatively, you can connect the headstage to the capillary outside the instrument, and manually inject the BGE into the capillary using a syringe connected to the capillary.
-# Click Zero in the Offset box.
+# Click Zero in the Offset box. It may be best to zero the signal with the CE high voltage turned on, as this can cause a significant jump in the C4D signal.
 # Click OK to exit the C4D Amplifier window, and OK again to exit the Hardware Settings window.
 # Click Start. If it's a new file, enter a file name and click Save.

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 # Ensure you have installed the latest version of PowerChrom software from [http://www.edaq.com/software-downloads Software Downloads]
 # Connect the computer to the C4D hardware, following the instructions in the [https://www.edaq.com/edaq-product-manuals manuals].
-# Connect the C4D headstage to your capillary electrophoresis instrument, following the instructions in the relevant [https://www.edaq.com/wiki/Application_Notes#C4D_Conductivity_Detector Application Note]
+# Connect the C4D hardware unit to the third-party CE instrument: connect the trigger cable and/or signal cable, depending on whether you are using eDAQ or third-party software. Follow the instructions in the relevant [https://www.edaq.com/wiki/Application_Notes#C4D_Conductivity_Detector Application Note]
 # Turn on the C4D hardware.
 # Open the PowerChrom software.
@@ Line 63: / Line 64: @@
 # Click Zero in the Offset box.
 # Click OK to exit the C4D Amplifier window, and OK again to exit the Hardware Settings window.
-# Click Start and enter a name for the file. Click Save.
+# Click Start. If it's a new file, enter a file name and click Save.
-# Click Inject, in the Manual Sampling box (shown in Figure 5.), when the sample is injected into the capillary.
+# Click Inject, in the Manual Sampling box (shown in Figure 5.), when the sample is injected into the capillary, unless you have installed the trigger cable to trigger automatically.
 # Click Stop, in the Manual Sampling box, when you have collected all of your peaks.
-# Repeat steps 15, 16 and 17 for each blank, calibration standard and sample you wish to analyse.
+# Repeat the three previous steps for each blank, calibration standard and sample you wish to analyse.
 # Analyse the data, by integrating the peaks and drawing a calibration graph, as shown in the PowerChrom software [https://www.edaq.com/edaq-product-manuals manual] and the [https://www.edaq.com/wiki/Screencast_Training_Videos#PowerChrom screencast training videos]

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 * C4D hardware unit, either:
 ** [http://www.edaq.com/ER225 ER225 C4D Data System] including [http://www.edaq.com/ES280 PowerChrom software] or
-** [http://www.edaq.com/ER815 ER815 C4D Detector] with third-party data acquisition system
+** [http://www.edaq.com/ER815 ER815 C4D Detector] with either a third-party data acquisition system or PowerChrom software.
 * [http://www.edaq.com/ET120 ET120 C4D Headstage].
 * [Http://www.edaq.com/EC020 EC020 Standard Test Solutions for C4D]:
-** BGE = 0.5M acetic acid
+** BGE = 0.5M acetic acid.
-** Sample = 1mM LiCl, KNO<sub>3</sub> and Na<sub>2</sub>SO<sub>4</sub> in deionised water
+** Sample = 1mM LiCl, KNO<sub>3</sub> and Na<sub>2</sub>SO<sub>4</sub> in deionised water.
 * Computer with the latest [http://www.edaq.com/ES280 PowerChrom software].
 * Capillary electrophoresis instrument.

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 # Ensure you have installed the latest version of PowerChrom software from [http://www.edaq.com/software-downloads Software Downloads]
 # Connect the computer to the C4D hardware, following the instructions in the [https://www.edaq.com/edaq-product-manuals manuals].
-# Connect the C4D headstage to your instrument, following the instructions in the relevant [https://www.edaq.com/wiki/Application_Notes#C4D_Conductivity_Detector Application Note]
+# Connect the C4D headstage to your capillary electrophoresis instrument, following the instructions in the relevant [https://www.edaq.com/wiki/Application_Notes#C4D_Conductivity_Detector Application Note]
 # Turn on the C4D hardware.
 # Open the PowerChrom software.

@@ Line 45: / Line 45: @@
 |}
 # Ensure you have installed the latest version of PowerChrom software from [http://www.edaq.com/software-downloads Software Downloads]
-# Connect the computer to the C4D hardware, following the instructions in the [http://www.edaq.com/locked/manuals.php manual (website registration required)].
+# Connect the computer to the C4D hardware, following the instructions in the [https://www.edaq.com/edaq-product-manuals manuals].
-# Connect the C4D headstage to your instrument, following the instructions in the relevant [http://wiki.edaq.com/index.php/Application_Notes#C4D_Conductivity_Detector Application Note]
+# Connect the C4D headstage to your instrument, following the instructions in the relevant [https://www.edaq.com/wiki/Application_Notes#C4D_Conductivity_Detector Application Note]
 # Turn on the C4D hardware.
 # Open the PowerChrom software.
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 #* Sampling speed = 20/s
 #* Channel 1: Input = Input 1, Range = 50 mV
-#* Channel 2: Input = Input 2, Range = 2 V
+#* Channel 2: Input = OFF (newer models of the ER225 are able to record a signal from an external detector, like a UV detector, in channel 2.
 # Under Channel 1, click C4D Amplifier.
 # Set the following values, as shown in Figure 4.:

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 # Click Stop, in the Manual Sampling box, when you have collected all of your peaks.
 # Repeat steps 15, 16 and 17 for each blank, calibration standard and sample you wish to analyse.
-# Analyse the data, by integrating the peaks and drawing a calibration graph, as shown in the PowerChrom software manual and screencast which you can download from www.edaq.com/screencasts.php
+# Analyse the data, by integrating the peaks and drawing a calibration graph, as shown in the PowerChrom software [https://www.edaq.com/edaq-product-manuals manuals] and the [https://www.edaq.com/wiki/Screencast_Training_Videos#PowerChrom screencast training videos]
 == Results ==

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 * Capillary electrophoresis instrument.
 * Instruction manual to the CE instrument.
-* Instruction [http://www.edaq.com/locked/manuals.php manual (website registration required)] to the C4D instrument.
+* Instruction [https://www.edaq.com/edaq-product-manuals manual] to the C4D hardware and software.
-* Application note showing how to connect the C4D to your CE instrument.
+* Application note found [https://www.edaq.com/wiki/Application_Notes#C4D_Conductivity_Detector here] showing how to connect the C4D to your CE instrument.
 == Conditions ==