Waters HPLC with PowerChrom - Revision history

Oliver at 06:36, 26 May 2014

2014-05-26T06:36:27Z

2013-07-11T06:16:36Z

2013-07-11T06:15:48Z

2013-07-11T06:14:31Z

2013-07-10T13:03:02Z

2013-07-10T13:02:28Z

2013-07-10T12:59:01Z

2013-07-10T12:55:56Z

2013-07-10T12:54:03Z

2013-07-08T14:24:35Z

Undo revision 965 by Oliver (talk)

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 === Introduction ===
-This application note describes how a Waters HPLC with two detectors and an injection valve, can be connected to either a PowerChrom or e-corder unit. The PowerChrom software is triggered to start recording by the injection valve, and displays the signal from each detector simultaneously. This can be used to upgrade a HPLC which is connected to an integrator.
+This application note describes how a Waters HPLC with two detectors and an injection valve, can be connected to either a [http://www.edaq.com/powerchrom-system PowerChrom] or e-corder unit. The PowerChrom software is triggered to start recording by the injection valve, and displays the signal from each detector simultaneously. This can be used to upgrade a HPLC which is connected to an integrator.
 === Equipment Required ===
 * Waters HPLC with Waters 430 Conductivity Detector and Waters 484 Tunable Absorbance Detector
-* [http://www.edaq.com/powerchrom-system ER280 PowerChrom System]
+* [http://www.edaq.com/powerchrom-system PowerChrom System]
 * 3 cables: twisted-pair cables with braided shield
 * A computer

@@ Line 10: / Line 10: @@
 * 3 cables: twisted-pair cables with braided shield
 * A computer
 === Waters 430 Conductivity Detector ===
@@ Line 16: / Line 21: @@
 === Waters 484 Tunable Absorbance Detector ===
 The absorbance detector is connected to Channel 2 of the PowerChrom unit using a similar cable, shown in Figure 3.
 [[File:Figure 4. Waters injection valve.jpg|x200px|thumb|left|Figure 4. Waters injection valve]]

@@ Line 1: / Line 1: @@
 [[File:Figure 1. The ER280 PowerChrom System connected to a Waters HPLC.jpg|x200px|thumb|right|Figure 1. The ER280 PowerChrom System connected to a Waters HPLC]]
 ''The PowerChrom System can record and analyse data from two detectors on a Waters HPLC.''
@@ Line 24: / Line 23: @@
 [[File:Figure 4. Waters injection valve.jpg|x200px|thumb|left|Figure 4. Waters injection valve]]
 === Injection Valve ===
 The two black cables from the injection valve are connected to PowerChrom unit’s TRIG + and –, see Figure 4. In the PowerChrom software, the Inject Settings is set to “Wait for Inject”. This setting is shown in Video 1. Turning the injection valve will then trigger the start of the recording.

@@ Line 17: / Line 17: @@
 === Waters 484 Tunable Absorbance Detector ===
 The absorbance detector is connected to Channel 2 of the PowerChrom unit using a similar cable, shown in Figure 3.
 {|
@@ Line 25: / Line 22: @@
 | [[File:Figure 3. Waters 484 Tunable Absorbance Detector.jpg|x200px|thumb|right|Figure 3. Waters 484 Tunable Absorbance Detector]]
 |}
-{|
-| [[File:Figure 4. Waters injection valve.jpg|x200px|thumb|right|Figure 4. Waters injection valve]]
+[[File:Figure 4. Waters injection valve.jpg|x200px|thumb|left|Figure 4. Waters injection valve]]
-|}
+=== Injection Valve ===

@@ Line 6: / Line 6: @@
 This application note describes how a Waters HPLC with two detectors and an injection valve, can be connected to either a PowerChrom or e-corder unit. The PowerChrom software is triggered to start recording by the injection valve, and displays the signal from each detector simultaneously. This can be used to upgrade a HPLC which is connected to an integrator.
-== Equipment Required ==
+=== Equipment Required ===
 * Waters HPLC with Waters 430 Conductivity Detector and Waters 484 Tunable Absorbance Detector
 * [http://www.edaq.com/powerchrom-system ER280 PowerChrom System]

@@ Line 5: / Line 5: @@
 === Waters 430 Conductivity Detector ===
-The conductivity detector is connected to Channel 1 of the PowerChrom unit using a twisted-pair cable with braided shield. The shield wire should be connected to either the COM connector of the PowerChrom unit, or to a ground connection on the detector, but not to both.
+The conductivity detector is connected to Channel 1 of the PowerChrom unit using a twisted-pair cable with braided shield, as shown in Figure 2. The shield wire should be connected to either the COM connector of the PowerChrom unit, or to a ground connection on the detector, but not to both.
 === Waters 484 Tunable Absorbance Detector ===
-The absorbance detector is connected to Channel 2 of the PowerChrom unit using a similar cable.
+The absorbance detector is connected to Channel 2 of the PowerChrom unit using a similar cable, shown in Figure 3.
 === Injection Valve ===
-The two black cables from the injection valve are connected to PowerChrom unit’s TRIG + and –. In the PowerChrom software, the Inject Settings is set to “Wait for Inject”. Turning the injection valve will then trigger the start of the recording.
+The two black cables from the injection valve are connected to PowerChrom unit’s TRIG + and –, see Figure 4. In the PowerChrom software, the Inject Settings is set to “Wait for Inject”. Turning the injection valve will then trigger the start of the recording.
 {|