Connecting the C4D to the WynSep Wyn-CE
How to connect the eDAQ C4D system to the WynSep Wyn-CE instrument.
Contents
Equipment Required
- C4D hardware unit, either:
- ER225 C4D Data System including PowerChrom software or
- ER815 C4D Detector to use the WynSep data acquisition software
- ET120 C4D Headstage for Capillary Electrophoresis
- WynSep Wyn-CE instrument
Choice of Software
The data from both C4D and UV-Visible detectors can be recorded and displayed in real time, using either the eDAQ PowerChrom software or the WynSep data acquisition software. Choose the:
- ER225 C4D Data System to use PowerChrom software. The analog output of the WynSep UV detector is connected to the analog input of the ER225. PowerChrom software records the data from both detectors.
- ER815 C4D Detector, to use WynSep data acquisition software. The analog output from the ER815 is connected to the WynSep instrument, so the WynSep data acquisition software records data from both detectors.
Installing the C4D Headstage in the WynSep Wyn-CE Cartridge
It’s easy to mount the C4D headstage inside the WynSep Wyn-CE cartridge. Simply thread the capillary through the channel in the side of the C4D headstage and fix in place.
You should ensure that the C4D headstage and its flat grey cable are at least 1 cm away from the capillary as it loops inside the cartridge. This is to prevent the high voltage inside the capillary from shorting through the headstage and its cable, which are both electrically grounded.
You should also ensure that the C4D hardware unit is connected to an electrical socket which is grounded. Connect the C4D hardware directly to the electrical socket in the wall, not using an electrical power board.
Applications
Comparison between C4D and UV Detectors for the Analysis of Organic Acids
The WynSep Wyn-CE was used to compare C4D and UV detectors when analysing organic acids in a standard solution and in red wine. The results are shown in Figure 1.
A comparison of the limits of detection of the organic acids, using the two detection methods, is shown in Figure 2.