Difference between revisions of "Anions by MCE-C4D"

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(Equipment and Reagents Required)
(Equipment and Reagents Required)
 
(9 intermediate revisions by the same user not shown)
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[[File:Chip diag.jpg|300px|thumb|right|]]
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[[File:Chip diag.jpg|300px|thumb|right|Figure 1. Chip nomenclature]]
[[File:Electropherogram of 100 µM Cl–, NO3– and HSO4–.jpg|300px|thumb|right|Figure 1. Electropherogram of 100 μM Cl–, NO3– and HSO4–.]]
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[[File:Electropherogram of 100 µM Cl–, NO3– and HSO4–.jpg|300px|thumb|right|Figure 2. Electropherogram of 100 μM Cl<sup>-</sup>, NO<sub>3</sub><sup>-</sup> and HSO<sub>4</sub><sup>-</sup>.]]
[[File:Electropherogram of 10 µM Cl–, NO3–, HSO4–.jpg|300px|thumb|right|Figure 2. Electropherogram of 10 μM Cl–, NO3–, HSO4–.]]
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[[File:Electropherogram of 10 µM Cl–, NO3–, HSO4–.jpg|300px|thumb|right|Figure 3. Electropherogram of 10 μM Cl<sup>-</sup>, NO<sub>3</sub><sup>-</sup>, HSO<sub>4</sub><sup>-</sup>.]]
  
 
''The eDAQ C4D system was used to detect three inorganic anions, separated by microchip electrophoresis, by using contactless conductivity detection.''
 
''The eDAQ C4D system was used to detect three inorganic anions, separated by microchip electrophoresis, by using contactless conductivity detection.''
  
 
== Introduction ==
 
== Introduction ==
The three inorganic anions Cl–, NO3– and HSO4– were separated using microchip electrophoresis (MCE). These anions were then detected using the eDAQ C4D system.  
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The three inorganic anions Cl<sup>-</sup>, NO<sub>3</sub><sup>-</sup> and HSO<sub>4</sub><sup>-</sup> were separated using microchip electrophoresis (MCE). These anions were then detected using the eDAQ C4D system.
  
 
== Equipment and Reagents Required ==
 
== Equipment and Reagents Required ==
* [Http://www.edaq.com/ER255 ER255] or [Http://www.edaq.com/ER455 ER455] Microchip Electrophoresis bundles, including:
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* [Http://www.edaq.com/ER455 ER455] Microchip Electrophoresis kit, including:
 
** [Http://www.edaq.com/ER225 ER225 C4D Data System]
 
** [Http://www.edaq.com/ER225 ER225 C4D Data System]
 
** [http://www.edaq.com/ET225 ET225 Micronit Platform]
 
** [http://www.edaq.com/ET225 ET225 Micronit Platform]
** [http://www.edaq.com/ER230_HV-Sequencer ER230 High Voltage Sequencer] (one or two units)
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** [http://www.edaq.com/ER430 ER430 High Voltage Sequencer]
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** [http://www.edaq.com/ES280 PowerChrom] and [http://www.edaq.com/locked/software/sequencer.php QuadSequencer] softwares
 
** [http://www.edaq.com/ET145-4 ET145-4 CE Microchip (45 mm) kit]
 
** [http://www.edaq.com/ET145-4 ET145-4 CE Microchip (45 mm) kit]
** [http://www.edaq.com/ES280 PowerChrom] and [http://www.edaq.com/locked/software/sequencer.php Sequencer] softwares
 
 
* Background Electrolyte (BGE) = 70 mM TRIS/CHES with 1ppm HDMB, where TRIS is Tris(hydroxymethyl)aminomethane, CHES is 2-(Cyclohexylamino)ethanesulfonic acid, and HDMB is hexadimethrine bromide  
 
* Background Electrolyte (BGE) = 70 mM TRIS/CHES with 1ppm HDMB, where TRIS is Tris(hydroxymethyl)aminomethane, CHES is 2-(Cyclohexylamino)ethanesulfonic acid, and HDMB is hexadimethrine bromide  
* Samples = 100 μM and 10 μM Cl–, NO3– and HSO4– in deionised water.
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* Samples = 100 μM and 10 μM Cl<sup>-</sup>, NO<sub>3</sub><sup>-</sup> and HSO<sub>4</sub><sup>-</sup> in deionised water.
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* The following sequence was used with the High Volage Sequencer:
  
 
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== Data Analysis and Limit of Detection ==
 
== Data Analysis and Limit of Detection ==
Figure 1 shows the electropherogram of the 100 μM Cl–, NO3– and HSO4– solution. In order to measure the limit of detection of the anions, a 10 μM solution of the same analytes was analyzed. The electropherogram is shown in Figure 2.  
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Figure 2 shows the electropherogram of the 100 μM Cl<sup>-</sup>, NO<sub>3</sub><sup>-</sup> and HSO<sub>4</sub><sup>-</sup> solution. In order to measure the limit of detection of the anions, a 10 μM solution of the same analytes was analyzed. The electropherogram is shown in Figure 3.  
  
Using the definition of limit of detection as equal to three times the standard deviation of signal (during four seconds before the first peak), the limit of detection is 3.7 μM for Cl– and NO3–, and 1.3 μM for HSO4–.
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Using the definition of limit of detection as equal to three times the standard deviation of signal (during four seconds before the first peak), the limit of detection is 3.7 μM for Cl<sup>-</sup> and NO<sub>3</sub><sup>-</sup>, and 1.3 μM for HSO<sub>4</sub><sup>-</sup>.

Latest revision as of 17:18, 24 August 2016

Figure 1. Chip nomenclature
Figure 2. Electropherogram of 100 μM Cl-, NO3- and HSO4-.
Figure 3. Electropherogram of 10 μM Cl-, NO3-, HSO4-.

The eDAQ C4D system was used to detect three inorganic anions, separated by microchip electrophoresis, by using contactless conductivity detection.

Introduction

The three inorganic anions Cl-, NO3- and HSO4- were separated using microchip electrophoresis (MCE). These anions were then detected using the eDAQ C4D system.

Equipment and Reagents Required

Time (s) Step sample BGE waster outlet
0-20 loading -1000V -700V 0V -1000V
20-60 separation -750V -1000 V -750V 0V

Data Analysis and Limit of Detection

Figure 2 shows the electropherogram of the 100 μM Cl-, NO3- and HSO4- solution. In order to measure the limit of detection of the anions, a 10 μM solution of the same analytes was analyzed. The electropherogram is shown in Figure 3.

Using the definition of limit of detection as equal to three times the standard deviation of signal (during four seconds before the first peak), the limit of detection is 3.7 μM for Cl- and NO3-, and 1.3 μM for HSO4-.