Difference between revisions of "Anions by MCE-C4D"

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(Equipment and Reagents Required)
(Equipment and Reagents Required)
Line 14: Line 14:
 
** [http://www.edaq.com/ER430 ER430 High Voltage Sequencer]
 
** [http://www.edaq.com/ER430 ER430 High Voltage Sequencer]
 
** [http://www.edaq.com/ES280 PowerChrom] and [http://www.edaq.com/locked/software/sequencer.php QuadSequencer] softwares
 
** [http://www.edaq.com/ES280 PowerChrom] and [http://www.edaq.com/locked/software/sequencer.php QuadSequencer] softwares
 +
* [http://www.edaq.com/ET145-4 ET145-4 CE Microchip (45 mm) kit]
 
* Background Electrolyte (BGE) = 70 mM TRIS/CHES with 1ppm HDMB, where TRIS is Tris(hydroxymethyl)aminomethane, CHES is 2-(Cyclohexylamino)ethanesulfonic acid, and HDMB is hexadimethrine bromide  
 
* Background Electrolyte (BGE) = 70 mM TRIS/CHES with 1ppm HDMB, where TRIS is Tris(hydroxymethyl)aminomethane, CHES is 2-(Cyclohexylamino)ethanesulfonic acid, and HDMB is hexadimethrine bromide  
 
* Samples = 100 μM and 10 μM Cl<sup>-</sup>, NO<sub>3</sub><sup>-</sup> and HSO<sub>4</sub><sup>-</sup> in deionised water.
 
* Samples = 100 μM and 10 μM Cl<sup>-</sup>, NO<sub>3</sub><sup>-</sup> and HSO<sub>4</sub><sup>-</sup> in deionised water.

Revision as of 18:49, 27 April 2016

Figure 1. Chip nomenclature
Figure 2. Electropherogram of 100 μM Cl-, NO3- and HSO4-.
Figure 3. Electropherogram of 10 μM Cl-, NO3-, HSO4-.

The eDAQ C4D system was used to detect three inorganic anions, separated by microchip electrophoresis, by using contactless conductivity detection.

Introduction

The three inorganic anions Cl-, NO3- and HSO4- were separated using microchip electrophoresis (MCE). These anions were then detected using the eDAQ C4D system.

Equipment and Reagents Required

Time (s) Step sample BGE waster outlet
0-20 loading -1000V -700V 0V -1000V
20-60 separation -750V -1000 V -750V 0V

Data Analysis and Limit of Detection

Figure 2 shows the electropherogram of the 100 μM Cl-, NO3- and HSO4- solution. In order to measure the limit of detection of the anions, a 10 μM solution of the same analytes was analyzed. The electropherogram is shown in Figure 3.

Using the definition of limit of detection as equal to three times the standard deviation of signal (during four seconds before the first peak), the limit of detection is 3.7 μM for Cl- and NO3-, and 1.3 μM for HSO4-.