Difference between revisions of "Appropriate Frequency to use with Different Background Electrolytes and Capillary Inner Diameters"

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Revision as of 17:41, 2 September 2014

The eDAQ C4D lets the user select C4D settings (frequency, amplitude and headstage gain). The optimal C4D settings depends on the background electrolyte (buffer) being used. You can download the C4D Profiler and read the instructions

This table indicates the appropriate frequency to use with different background electrolytes and capillary inner diameters. The C4D Profiler should find that the optimum frequency is in the range of the cells with green shading, while the red shaded cells are outside the frequency limits of the instrument.


Capillary ID (µm) Background Electrolyte Conductivity (µS/cm)
<25 25-100 100-400 400-800 800-1600 1600-3200
25 <50 <50 <50 100 200 400
50 <50 <50 100 200 600 1200
75 <50 80 200 600 1200 2000
100 60 100 400 1000 1500 >2000
150 80 200 800 1200 1800 >2000
200 100 300 1000 1500 >2000 >2000
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