Difference between revisions of "Appropriate Frequency to use with Different Background Electrolytes and Capillary Inner Diameters"
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Revision as of 17:27, 1 September 2014
This table indicates the appropriate frequency (kHz) to use with different buffers and different capillary inner diameters (IDs).
The C4D Profiler will confirm this choice and allow the selection of C4D settings (frequency, amplitude and headstage gain) that provides optimum signal levels.
| Capillary ID (µm) | Buffer Conductivity (µS/cm) | |||||
|---|---|---|---|---|---|---|
| <25 | 25-100 | 100- 400 | 400-800 | 800-1600 | 1600-3200 | |
| 25 | <50 | <50 | <50 | 100 | 200 | 400 |
| 50 | <50 | <50 | 100 | 200 | 600 | 1200 |
| 75 | <50 | 80 | 200 | 600 | 1200 | 2000 |
| 100 | 60 | 100 | 400 | 1000 | 1500 | >2000 |
| 150 | 80 | 200 | 800 | 1200 | 1800 | >2000 |
| 200 | 100 | 300 | 1000 | 1500 | >2000 | >2000 |
