Difference between revisions of "Appropriate Frequency to use with Different Background Electrolytes and Capillary Inner Diameters"
From eDAQ Wiki
(Created page with "This table indicates the appropriate frequency (kHz) to use with different buffers and different capillary inner diameters (IDs). The C4D Profiler will confirm this choice a...") |
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− | + | The eDAQ C4D lets the user select C4D settings (frequency, amplitude and headstage gain). The optimal C4D settings depends on the background electrolyte (buffer) being used. You can [http://www.edaq.com/software-downloads download the C4D Profiler] and [http://wiki.edaq.com/index.php/C4D_Profiler_in_PowerChrom_Software read the instructions] | |
− | + | This table indicates the appropriate frequency to use with different background electrolytes and capillary inner diameters. | |
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|- | |- | ||
! Capillary ID (µm) | ! Capillary ID (µm) | ||
− | ! colspan="6" | | + | ! colspan="6" | Background Electrolyte Conductivity (µS/cm) |
|- | |- | ||
! | ! | ||
! scope="col" width="70px" | <25 | ! scope="col" width="70px" | <25 | ||
! scope="col" width="70px" | 25-100 | ! scope="col" width="70px" | 25-100 | ||
− | ! scope="col" width="70px" | 100- 400 | + | ! scope="col" width="70px" | 100-400 |
! scope="col" width="70px" | 400-800 | ! scope="col" width="70px" | 400-800 | ||
! scope="col" width="70px" | 800-1600 | ! scope="col" width="70px" | 800-1600 |
Revision as of 17:31, 1 September 2014
The eDAQ C4D lets the user select C4D settings (frequency, amplitude and headstage gain). The optimal C4D settings depends on the background electrolyte (buffer) being used. You can download the C4D Profiler and read the instructions
This table indicates the appropriate frequency to use with different background electrolytes and capillary inner diameters.
Capillary ID (µm) | Background Electrolyte Conductivity (µS/cm) | |||||
---|---|---|---|---|---|---|
<25 | 25-100 | 100-400 | 400-800 | 800-1600 | 1600-3200 | |
25 | <50 | <50 | <50 | 100 | 200 | 400 |
50 | <50 | <50 | 100 | 200 | 600 | 1200 |
75 | <50 | 80 | 200 | 600 | 1200 | 2000 |
100 | 60 | 100 | 400 | 1000 | 1500 | >2000 |
150 | 80 | 200 | 800 | 1200 | 1800 | >2000 |
200 | 100 | 300 | 1000 | 1500 | >2000 | >2000 |