Appropriate Frequency to use with Different Background Electrolytes and Capillary Inner Diameters

The eDAQ C4D lets the user select C4D settings (frequency, amplitude and headstage gain). The optimal C4D settings depends on the background electrolyte (buffer) being used. You can download the C4D Profiler and read the instructions

This table indicates the appropriate frequency to use with different background electrolytes and capillary inner diameters.

Capillary ID (µm)	Background Electrolyte Conductivity (µS/cm)
	<25	25-100	100-400	400-800	800-1600	1600-3200
25	<50	<50	<50	100	200	400
50	<50	<50	100	200	600	1200
75	<50	80	200	600	1200	2000
100	60	100	400	1000	1500	>2000
150	80	200	800	1200	1800	>2000
200	100	300	1000	1500	>2000	>2000