Difference between revisions of "C4Dヘッドステージ"
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[[File:Ions 150um.jpg|300px|thumb|right|三種類の陽イオン]] | [[File:Ions 150um.jpg|300px|thumb|right|三種類の陽イオン]] | ||
| − | '' | + | ''この例では、C4D (容量結合型非接触電導度検出器) ヘッドステージは外径 150 μm のキャピラリーチューブ用で三種類のカチオンを検出します。'' |
| − | == | + | == '''はじめに''' == |
eDAQ has developed a C4D headstage for use with capillaries with an outer diameter of 150 μm. The headstage was used for the analysis of a solution containing potassium, sodium and lithium chlorides dissolved in MES/His background electrolyte (BGE). | eDAQ has developed a C4D headstage for use with capillaries with an outer diameter of 150 μm. The headstage was used for the analysis of a solution containing potassium, sodium and lithium chlorides dissolved in MES/His background electrolyte (BGE). | ||
The headstage can be used with any eDAQ C4D hardware. | The headstage can be used with any eDAQ C4D hardware. | ||
Revision as of 13:05, 20 February 2014
File:EDAQ 150 μm C4D ヘッドステージ.jpg
eDAQ 150 μm C4D ヘッドステージ
この例では、C4D (容量結合型非接触電導度検出器) ヘッドステージは外径 150 μm のキャピラリーチューブ用で三種類のカチオンを検出します。
はじめに
eDAQ has developed a C4D headstage for use with capillaries with an outer diameter of 150 μm. The headstage was used for the analysis of a solution containing potassium, sodium and lithium chlorides dissolved in MES/His background electrolyte (BGE). The headstage can be used with any eDAQ C4D hardware.
A limit of detection of 3 µM was obtained.
Experimental
- Capillary from Polymicro Technologies, 150 µm OD, 76 µm ID, 60 cm long.
- Sample: 200 µM Na, K, Li (as chloride salts) dissolved in water and BGE
- BGE: 20 mM of 2-(N-morpholino)ethanesulfonic acid and L-histidine
- The new capillary was prepared by flushing with 0.1 M HCl, water, 0.1 M NaOH, water, then BGE, for 5 minutes each
- Instrument: built in-house at University of Basel
- Injection: 10 s siphoning at 10 cm height
- Separation voltage: 25 kV (10 μA current)
- C4D settings: 700 kHz, 100 V, gain ON
