C4Dマイクロチップ電気泳動のアニオン検出
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Revision as of 12:40, 28 February 2014 by Mori1240 (Talk | contribs) (→Equipment and Reagents Required)
非接触電導度検出器を用いてマイクロチップ電気泳動によって三種類の無機アニオンの検出に eDAQ C4D システムが使われています。
はじめに
三種類の無機陰イオン Cl-, NO3- 、及び HSO4- をマイクロチップ電気泳動法 (MCE) を使って分離し、 eDAQ C4D システムで検出します。
必要な装置と試薬
- ER255 または ER455 マイクロチップ電気泳動システム、システムの構成 :
- ER225 C4D データシステム
- ET225 マイクロチッププラットフォームj
- ER230 High Voltage Sequencer (one or two units)
- ET145-4 CE Microchip (45 mm) kit
- PowerChrom and Sequencer softwares
- Background Electrolyte (BGE) = 70 mM TRIS/CHES with 1ppm HDMB, where TRIS is Tris(hydroxymethyl)aminomethane, CHES is 2-(Cyclohexylamino)ethanesulfonic acid, and HDMB is hexadimethrine bromide
- Samples = 100 μM and 10 μM Cl-, NO3- and HSO4- in deionised water.
- The following sequence was used with the High Volage Sequencer:
Time (s) | Step | sample | BGE | waster | outlet |
---|---|---|---|---|---|
0-20 | loading | -1000V | -700V | 0V | -1000V |
20-60 | separation | -750V | -1000 V | -750V | 0V |
Data Analysis and Limit of Detection
Figure 2 shows the electropherogram of the 100 μM Cl-, NO3- and HSO4- solution. In order to measure the limit of detection of the anions, a 10 μM solution of the same analytes was analyzed. The electropherogram is shown in Figure 3.
Using the definition of limit of detection as equal to three times the standard deviation of signal (during four seconds before the first peak), the limit of detection is 3.7 μM for Cl- and NO3-, and 1.3 μM for HSO4-.