Difference between revisions of "C4D Analysis of Acetylchorine Chloride on a Prince CE System"
From eDAQ Wiki
(→Conditions) |
(→Introduction) |
||
(2 intermediate revisions by the same user not shown) | |||
Line 5: | Line 5: | ||
== Introduction == | == Introduction == | ||
− | The eDAQ C4D system was connected to the PrinCE-C 600 capillary electrophoresis (CE) instrument as described in the application note C4D004. Acetylcholine chloride (AChCl) was then analysed and a calibration curve was produced. The CE instrument was set to negative mode and the C4D system measured the Cl- anions. | + | The eDAQ C4D system was connected to the PrinCE-C 600 capillary electrophoresis (CE) instrument as described in the application note C4D004. Acetylcholine chloride (AChCl) was then analysed and a calibration curve was produced. The CE instrument was set to negative mode and the C4D system measured the Cl<sup>-</sup> anions. |
== Conditions == | == Conditions == | ||
Line 20: | Line 20: | ||
** length to detector = 42 cm | ** length to detector = 42 cm | ||
* Injection: pressure 300 mbar for 10 seconds | * Injection: pressure 300 mbar for 10 seconds | ||
− | * C4D | + | * C4D settings |
** frequency = 500 kHz | ** frequency = 500 kHz | ||
** amplitude = 100 A | ** amplitude = 100 A | ||
** headstage gain OFF | ** headstage gain OFF | ||
* Data recording: | * Data recording: | ||
− | ** lowpass | + | ** lowpass filter = 1 Hz |
** range = 50 mV | ** range = 50 mV | ||
** sampling rate = 100 data points per second | ** sampling rate = 100 data points per second |
Latest revision as of 16:11, 7 January 2014
The eDAQ C4D system was connected to the PrinCE-C 600 capillary electrophoresis instrument and acetylcholine chloride was analysed.
Introduction
The eDAQ C4D system was connected to the PrinCE-C 600 capillary electrophoresis (CE) instrument as described in the application note C4D004. Acetylcholine chloride (AChCl) was then analysed and a calibration curve was produced. The CE instrument was set to negative mode and the C4D system measured the Cl- anions.
Conditions
- Analyte: Acetylcholine chloride (AChCl) dissolved in water, analysed at the concentrations 10 mM, 50 mM, 100 mM, 500 mM and 1000 mM
- Buffer:
- MES/Arg/CTAB with pH 5.7, where MES is 2-(N-morpholino)ethanesulfonic acid, Arg is L(+)-Arginine, and CTAB is hexadecyltrimethylammonium bromide
- Instrument:
- separation voltage = 30 kV
- recorded separation current = 11 – 12 µA
- Capillary:
- outer diameter = 360 µm
- internal diameter = 25 µm
- length = 56 cm
- length to detector = 42 cm
- Injection: pressure 300 mbar for 10 seconds
- C4D settings
- frequency = 500 kHz
- amplitude = 100 A
- headstage gain OFF
- Data recording:
- lowpass filter = 1 Hz
- range = 50 mV
- sampling rate = 100 data points per second
Data Analysis
Figure 1 shows the AChCl peaks at the different concentrations. Figure 2 shows how the software can automatically produce a calibration curve by plotting the peak areas versus known concentrations of analyte.