Connecting the C4D to the Beckman Coulter CE

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eDAQ C4D with Beckman Coulter PA 800plus
C4D headstage connected to the capillary

This application note describes how to connect the eDAQ C4D system to the P/ACE MDQ and PA 800 plus capillary electrophoresis instruments from Beckman Coulter.

Introduction

The eDAQ C4D can be connected to the Beckman Coulter CE instruments, providing contactless conductivity detection for the instrument. Both the C4D headstage and UV detector can be connected to the capillary at the same time, enabling both types of detection to be performed with a single sample injection.

Choice of Software

The data from both the eDAQ C4D and Beckman Coulter UV/LIF/PDA detectors can be displayed in real time, using either the eDAQ software or the Beckman Coulter PA 800 plus Software. Choose:

  • ER225 C4D Data System, to use the eDAQ software. The analog output from the UV/LIF/PDA detector (on the CE) may be connected to the eDAQ system, so the eDAQ software records data from both detectors.
  • ER125 C4D Detector, to use Beckman Coulter software. The analog output from the C4D may be connected to the CE, so the PA 800 plus Software records data from both detectors. This may require an external A/D interface. The delta K signal from the C4D Detector should be connected to the A/D interface input. The A/D interface is then connected to the computer using a serial connection.
Analog output channels on CE instrument.

Analog Outputs on the CE

There are three BNC analog outputs located on the left side panel of the CE instrument, as shown in Figure 3. The outputs are 1000 mV full scale deflection. For PA 800 plus firmware version 1.5 or higher, the output is programmable from the control software. In version 1.2, 1000 mV is equal to 1 absorbance unit (AU) with a fixed 10% negative offset. The high voltage output is the uncontrolled parameter, current or voltage, of the high voltage power supply. In this instance, 1000 mV is equal to 30,000 V or 300 µA in all firmware versions.