Difference between revisions of "ET125ヘッドステージを用いたC4Dイオンクロマトグラフィー"
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| − | [[File: | + | [[File:DSC06289.JPG|300px|thumb|right|Figure 1. ET125 Headstage on 1/16th inch tubing after the column]] |
| − | [[File: | + | [[File:Chromatogram of 1mM anions.jpg|300px|thumb|right|Figure 2. Chromatogram of 1mM anions]] |
| − | ''eDAQ C4D | + | ''The eDAQ C4D system was connected directly to 1/16th inch tubing for IC/HPLC and used to detect six anions.'' |
| − | == | + | == Introduction == |
| − | + | The eDAQ [http://www.edaq.com/ET125 ET125 General Purpose C4D Headstage] is a contactless conductivity detector for ion chromatography (IC) and high-performance liquid chromatography (HPLC) instruments. It is designed to connect directly to tubing sizes 1/8 inch (3.2 mm) OD or less (the users specifies the OD when ordering the product). For example, the 1/16th inch (1.6 mm) outer diameter headstage connects directly to the standard for tubing for IC and HPLC. | |
| − | + | Connecting the detector immediately after the separation column, as shown in Figure 1, greatly reduces extra-column band broadening (remixing of the analytes after the column) because the “dead volume” is reduced. | |
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| − | == | + | A sample containing 1 mM fluoride, chloride, nitrate, bromide, pyruvate and nitrate was analysed; the chromatogram is shown in Figure 2. |
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| − | + | The method used was similar to experiments previously published, except contactless (as opposed to contact) conductivity detection was used: Separation of anions by ion-interaction chromatography with a novel cationic/zwitterionic eluent; Zhu Yan, Ling Yanyan, James S. Fritz, Paul R. Haddad; Journal of Chromatography A, 1020 (2003) 259–264 | |
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| + | == Equipment Required == | ||
| + | * C4D hardware unit, either: | ||
| + | ** [http://www.edaq.com/ER225 ER225 C4D Data System] including [http://www.edaq.com/ES280 PowerChrom software] | ||
| + | ** [http://www.edaq.com/ER815 ER815 C4D Detector] with third-party data acquisition system | ||
| + | * [http://www.edaq.com/ET125 ET125 General Purpose C4D Headstage] | ||
| + | * HPLC instrument | ||
| + | |||
| + | == Conditions == | ||
| + | * Sample: A solution of 1 mM fluoride, chloride, nitrate, bromide, pyruvate and nitrate, dissolved in water. | ||
| + | * Buffer: 98.75% water, 1.25% methanol, 2.5mM tetrabutylammonium hydroxide (TBAOH) and 5mM 3-(N-morpholino)-propane sulfonic acid (MOPS). | ||
| + | * Separation column: Macherey-Nagel, Nucleosil 120.5 C18, part 720041.46. | ||
| + | * C4D settings | ||
| + | ** frequency = 800 kHz | ||
| + | ** amplitude = 80 A | ||
| + | ** headstage gain OFF. | ||
| + | * Data recording: | ||
| + | ** lowpass Filter = 10 Hz | ||
| + | ** range = 20 mV | ||
| + | ** sampling rate = 1000 data points per second. | ||
Revision as of 13:22, 21 February 2014
The eDAQ C4D system was connected directly to 1/16th inch tubing for IC/HPLC and used to detect six anions.
Introduction
The eDAQ ET125 General Purpose C4D Headstage is a contactless conductivity detector for ion chromatography (IC) and high-performance liquid chromatography (HPLC) instruments. It is designed to connect directly to tubing sizes 1/8 inch (3.2 mm) OD or less (the users specifies the OD when ordering the product). For example, the 1/16th inch (1.6 mm) outer diameter headstage connects directly to the standard for tubing for IC and HPLC.
Connecting the detector immediately after the separation column, as shown in Figure 1, greatly reduces extra-column band broadening (remixing of the analytes after the column) because the “dead volume” is reduced.
A sample containing 1 mM fluoride, chloride, nitrate, bromide, pyruvate and nitrate was analysed; the chromatogram is shown in Figure 2.
The method used was similar to experiments previously published, except contactless (as opposed to contact) conductivity detection was used: Separation of anions by ion-interaction chromatography with a novel cationic/zwitterionic eluent; Zhu Yan, Ling Yanyan, James S. Fritz, Paul R. Haddad; Journal of Chromatography A, 1020 (2003) 259–264
Equipment Required
- C4D hardware unit, either:
- ER225 C4D Data System including PowerChrom software
- ER815 C4D Detector with third-party data acquisition system
- ET125 General Purpose C4D Headstage
- HPLC instrument
Conditions
- Sample: A solution of 1 mM fluoride, chloride, nitrate, bromide, pyruvate and nitrate, dissolved in water.
- Buffer: 98.75% water, 1.25% methanol, 2.5mM tetrabutylammonium hydroxide (TBAOH) and 5mM 3-(N-morpholino)-propane sulfonic acid (MOPS).
- Separation column: Macherey-Nagel, Nucleosil 120.5 C18, part 720041.46.
- C4D settings
- frequency = 800 kHz
- amplitude = 80 A
- headstage gain OFF.
- Data recording:
- lowpass Filter = 10 Hz
- range = 20 mV
- sampling rate = 1000 data points per second.
