Difference between revisions of "Measuring the Conductivity of a Liquid in a Pipette Tip"

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''The conductivity of a solution inside a pipette tip can be measured using the [https://www.edaq.com/ER818 ER818] Octal Contactless Conductivity System.''
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''The conductivity of a liquid inside a pipette tip can be measured using the [https://www.edaq.com/ER818 ER818] Octal Contactless Conductivity System.''
  
 
=== Introduction ===
 
=== Introduction ===
  
The ER818A system was developed to enable the measurement of conductivity of samples with the following general characteristics:
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The ER818 offers a method to measure the conductivity of a liquid inside a pipette tip. This can be integrated into an automated robotic system, which delivers the pipette tip into the Octal headstage. Measuring the conductivity directly in the pipette tip, instead of transferring the liquid to a measuring vessel, has a number of advantages:
  
 
* Low sample volumes
 
* Low sample volumes
 
* Low sample wastage
 
* Low sample wastage
 
* Zero cross-contamination
 
* Zero cross-contamination
* 100 µS/cm to 20,000 µS/cm conductivity range
 
* Ready operation in an automated system
 
  
[[File:Sample tube types C4D ER818 ET128.jpg|200px|thumb|right|Figure 1. Typical sample mediums]]
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The system has a conductivity range typically of 100 µS/cm to 10,000 µS/cm (the range is determined by the inner diameter of the sample tube).
  
  
Typical sample mediums are shown in Figure 1. All are based on individual samples, except for the 1600 µm outer diameter (OD) capillary, which allows for either a continuous or sampled liquid to be monitored.
 
  
1 Straight tube tip
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[[File:Hamilton pipette tip inside C4D ER818 ET128 hor.jpg|300px|thumb|left|Hamilton pipette tip and the positioning of the C4D electrodes (in red)]]
  
2 Tapered tube tip
 
  
3 Capillary tubing with 1600 µm OD
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The system uses a [https://www.edaq.com/c4d-contactless-conductivity-introduction capacitively-coupled contactless conductivity] measurement (C4D). This applies a high frequency signal to an excitation electrode on the outside of the pipette tip. This couples the signal to the sample by capacitive-coupling. A detection electrode, located some distance from the sample, measures the resulting current. The current is proportional to the sample conductivity. The measured quantity will depend on the physical dimensions of the test volume and its conductivity.
  
4 Melting point disposable glass tube with 1600 µm OD
 
  
5 NMR tube with 5mm OD
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[[File:Sample tube types C4D ER818 ET128.jpg|200px|thumb|right|Typical sample mediums]]
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'''Other possible sample mediums.''' Typical sample mediums are shown in the figure. All are based on individual samples, except for the 1600 µm outer diameter (OD) capillary, which also allows for a continuous conductivity measurement to be made as the sample flow through the capillary.
  
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1. Straight pipette tip
  
[[File:Hamilton pipette tip inside C4D ER818 ET128 hor.jpg|300px|thumb|left|Figure 2. Hamilton pipette tip and the positioning of the C4D electrodes]]
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2. Tapered pipette tip
The system uses a [https://www.edaq.com/c4d-contactless-conductivity-introduction capacitively-coupled contactless conductivity] measurement (C4D). This applies a high frequency signal to an excitation electrode which couples the signal to the sample. A detection electrode located some distance from the sample measures the resulting current, which is proportional to the sample conductivity. The measured quantity will depend on the physical dimensions of the test volume and its conductivity.
+
  
 +
3. Capillary tubing with 1600 µm OD
  
[[File:Setup C4D ER818 ET128.jpg|300px|thumb|right|Figure 3. Equipment setup with ER815 and ET128]]
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4. Melting point disposable glass tube with 1600 µm OD
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 +
5. NMR tube with 5 mm OD
 +
 
 +
 
 +
[[File:Setup C4D ER818 ET128.jpg|300px|thumb|right|Equipment setup with ER815 and ET128]]
 
=== Equipment Required ===
 
=== Equipment Required ===
 
* [https://www.edaq.com/ER818 ER818] Octal Contactless Conductivity System, which includes:
 
* [https://www.edaq.com/ER818 ER818] Octal Contactless Conductivity System, which includes:
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* Your pipette tips with samples
 
* Your pipette tips with samples
 
* A standard solution with conductivity in the middle of the measuring range, say 5 mS/cm
 
* A standard solution with conductivity in the middle of the measuring range, say 5 mS/cm
 +
  
 
=== Experiment Procedure ===
 
=== Experiment Procedure ===
A Quick Start guide describes how to connect the system, how to use Tera Term software to calibrate the system using a standard solution, adjust the gain and measure the conductivity of an unknown sample. The Quick Start guide can be downloaded from here.
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A Quick Start guide describes how to connect the system, how to use Tera Term software to calibrate the system using a standard solution, how to adjust the gain and measure the conductivity of an unknown sample. The Quick Start guide can be downloaded from the [https://www.edaq.com/edaq-product-manuals manuals webpage].
  
  
 
=== Notes ===
 
=== Notes ===
==== Important Note about Temperature ====
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==== Temperature ====
The volumes of liquid involved in these measurements are very small and subject to temperature variation.  Typically there will be a 4 to 5 °C temperature difference between the ambient laboratory air and the internal temperature of the headstage.  This corresponds to a potential error of approximately 5-6%.
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The volumes of the liquids involved in these measurements are very small and subject to temperature variation.  Typically there will be a 4 to 5 °C temperature difference between the ambient laboratory air and the internal temperature of the ET128 Octal headstage.  This corresponds to a potential error in conductivity measurement of approximately 5-6%. Since the internal temperature of the headstage is available, temperature compensation can be performed.
  
Since the internal temperature of the headstage is available – some temperature compensation can be performed.
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Allowing some time for the temperature to equalise can also help in reducing the temperature error. Another approach is to make the conductivity measurement as quickly as possible, before the sample temperature increases.
 
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Allowing some time for the temperature to equalise can also help in reducing the temperature error. Another approach could be to measure the conductivity as quickly as possible before the sample temperature increases.
+
  
 
==== Use of a Reference Channel ====
 
==== Use of a Reference Channel ====
  
In some applications where small changes in conductivity are to be measured, a reference channel is provided which operates as follows.
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In some applications where small changes in conductivity are to be measured, a reference channel can be used increase the sensitivity of the system and allow for temperature compensation. For example, if the conductivity of the unknown solutions are in the range 2 to 4 mS/cm, a reference solution of 3 mS/cm is selected and placed in the reference channel of the ET128 Octal headstage. This subtracts the reference conductivity value from the unknown solution's conductivity value, so that only the difference is displayed.  An advantage of this technique is that any temperature drift in the reference will be removed from the unknown solution, thereby providing temperature compensation.
 
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A reference solution is selected, say 3 mS/cm. The unknown solutions to be tested have a conductivity of say 3 mS/cm +/- 1 mS/cm.  By selecting the reference solution, it effectively subtracts this value from the unknown solutions so that only the changes are displayed.  An advantage of this technique is that any temperature drift in the reference will be removed from the unknown solutions, thereby providing temperature compensation.
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Revision as of 10:32, 7 December 2017

The conductivity of a liquid inside a pipette tip can be measured using the ER818 Octal Contactless Conductivity System.

Introduction

The ER818 offers a method to measure the conductivity of a liquid inside a pipette tip. This can be integrated into an automated robotic system, which delivers the pipette tip into the Octal headstage. Measuring the conductivity directly in the pipette tip, instead of transferring the liquid to a measuring vessel, has a number of advantages:

  • Low sample volumes
  • Low sample wastage
  • Zero cross-contamination

The system has a conductivity range typically of 100 µS/cm to 10,000 µS/cm (the range is determined by the inner diameter of the sample tube).


Hamilton pipette tip and the positioning of the C4D electrodes (in red)


The system uses a capacitively-coupled contactless conductivity measurement (C4D). This applies a high frequency signal to an excitation electrode on the outside of the pipette tip. This couples the signal to the sample by capacitive-coupling. A detection electrode, located some distance from the sample, measures the resulting current. The current is proportional to the sample conductivity. The measured quantity will depend on the physical dimensions of the test volume and its conductivity.


Typical sample mediums

Other possible sample mediums. Typical sample mediums are shown in the figure. All are based on individual samples, except for the 1600 µm outer diameter (OD) capillary, which also allows for a continuous conductivity measurement to be made as the sample flow through the capillary.

1. Straight pipette tip

2. Tapered pipette tip

3. Capillary tubing with 1600 µm OD

4. Melting point disposable glass tube with 1600 µm OD

5. NMR tube with 5 mm OD


Equipment setup with ER815 and ET128

Equipment Required

  • ER818 Octal Contactless Conductivity System, which includes:
    • ER815 Contactless Conductivity Detector
    • ET128 Octal headstage
  • A computer with your own software to record the data (for example, Tera Term, LabVIEW, C#, WinWedge or HyperACCESS)
  • Your pipette tips with samples
  • A standard solution with conductivity in the middle of the measuring range, say 5 mS/cm


Experiment Procedure

A Quick Start guide describes how to connect the system, how to use Tera Term software to calibrate the system using a standard solution, how to adjust the gain and measure the conductivity of an unknown sample. The Quick Start guide can be downloaded from the manuals webpage.


Notes

Temperature

The volumes of the liquids involved in these measurements are very small and subject to temperature variation. Typically there will be a 4 to 5 °C temperature difference between the ambient laboratory air and the internal temperature of the ET128 Octal headstage. This corresponds to a potential error in conductivity measurement of approximately 5-6%. Since the internal temperature of the headstage is available, temperature compensation can be performed.

Allowing some time for the temperature to equalise can also help in reducing the temperature error. Another approach is to make the conductivity measurement as quickly as possible, before the sample temperature increases.

Use of a Reference Channel

In some applications where small changes in conductivity are to be measured, a reference channel can be used increase the sensitivity of the system and allow for temperature compensation. For example, if the conductivity of the unknown solutions are in the range 2 to 4 mS/cm, a reference solution of 3 mS/cm is selected and placed in the reference channel of the ET128 Octal headstage. This subtracts the reference conductivity value from the unknown solution's conductivity value, so that only the difference is displayed. An advantage of this technique is that any temperature drift in the reference will be removed from the unknown solution, thereby providing temperature compensation.