Difference between revisions of "Waters HPLC with PowerChrom"

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(Injection Valve)
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[[File:Figure 4. Waters injection valve.jpg|300px|thumb|right|Figure 4. Waters injection valve]]
 
 
=== Injection Valve ===
 
=== Injection Valve ===
 
The two black cables from the injection valve are connected to PowerChrom unit’s TRIG + and –. In the PowerChrom software, the Inject Settings is set to “Wait for Inject”. Turning the injection valve will then trigger the start of the recording.
 
The two black cables from the injection valve are connected to PowerChrom unit’s TRIG + and –. In the PowerChrom software, the Inject Settings is set to “Wait for Inject”. Turning the injection valve will then trigger the start of the recording.
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[[File:Figure 4. Waters injection valve.jpg|x200px|thumb|right|Figure 4. Waters injection valve]]

Revision as of 01:23, 9 July 2013

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Figure 1. The ER280 PowerChrom System connected to a Waters HPLC

The PowerChrom System can record and analyse data from two detectors on a Waters HPLC.

Introduction

This application note describes how a Waters HPLC with two detectors and an injection valve, can be connected to either a PowerChrom or e-corder unit. The PowerChrom software is triggered to start recording by the injection valve, and displays the signal from each detector simultaneously. This can be used to upgrade a HPLC which is connected to an integrator.

Waters 430 Conductivity Detector

The conductivity detector is connected to Channel 1 of the PowerChrom unit using a twisted-pair cable with braided shield. The shield wire should be connected to either the COM connector of the PowerChrom unit, or to a ground connection on the detector, but not to both.

Waters 484 Tunable Absorbance Detector

The absorbance detector is connected to Channel 2 of the PowerChrom unit using a similar cable.

Figure 2. Waters 430 Conductivity Detector
Figure 3. Waters 484 Tunable Absorbance Detector

Injection Valve

The two black cables from the injection valve are connected to PowerChrom unit’s TRIG + and –. In the PowerChrom software, the Inject Settings is set to “Wait for Inject”. Turning the injection valve will then trigger the start of the recording.

Figure 4. Waters injection valve