Nitric oxide (NO) is a short-lived small gas molecule that is found in vivo where it acts as a signalling molecule. The study of nitric oxide and its derivatives is a subject of much active research.
'Clark' style polarographic electrodes and a suitable electrochemical meter are most often used for measurements in solution when continuous monitoring is required. Nitric oxide is usually oxidised to nitrate at the anode of the electrode probe. Most nitric oxide electrodes can be montored with e-corder and the:
Adjust the polarisation (poise voltage) from about +0.7 to +0.8 volts for optimum results. For examples see:
- Real-time electrochemical detection of extracellular nitric oxide in tobacco cells exposed to cryptogein, an elicitor of defence responses.
Angélique Besson-Bard, Sophie Griveau, Fethi Bediou,and David Wendehenne, Journal of Experimental Botany, 59, 3407-3414, 2008.
- In Vivo Electrochemical Detection of Nitroglycerin-Derived Nitric Oxide in Tumor-Bearing Mice. Sophie Griveau, Johanne Seguin, Daniel Scherman, Guy G. Chabot, Fethi Bedioui, Electroanalysis, Volume 21, 631-634, 2009.
Electrodes (and meters) are made by:
Remember to ask if the meter has analog output signal (which is suitable for connection to e-corder).
Chemiluminescence detectors can be use to detect nitric oxide in air or liquids. Compatible chemiluminesence analyzers are:
- GE Analytical Instruments Sievers Nitric Oxide Analyzer
- EcoMedics CLD 88 Analyzer. This instrument measures nitric oxide and its reaction products, nitrite, nitrate and nitrosothiols in virtually any biological fluid. Chart software with e-corder is usually the best choice for recording the signal.
It is also possible to determine nitric oxide concentration spectroscopically with the Eicom ENO-20 NOx analysis system. The reaction of nitric oxide with with an amine is used to produce a highly coloured molecule, the absorbance signal of which is monitored with a chromatography detector using a
If a flow analysis type system is being used to record a nitic oxide signal which comprises a sreies of peaks then the Chart software together with the FIA Chart Extension can be used to automatically detect and calibrate peaks. Calibration by peak area or peak height is possible and there are several curve fitting functions available. Results are displayed in user configurable reports.
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