Difference between revisions of "Waters HPLC with PowerChrom"
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[[File:Figure 1. The ER280 PowerChrom System connected to a Waters HPLC.jpg|x200px|thumb|right|Figure 1. The ER280 PowerChrom System connected to a Waters HPLC]] | [[File:Figure 1. The ER280 PowerChrom System connected to a Waters HPLC.jpg|x200px|thumb|right|Figure 1. The ER280 PowerChrom System connected to a Waters HPLC]] | ||
+ | {{#ev:youtube|SFNBn6TSjR0|300|right|Video 1. Basic Settings in PowerChrom}} | ||
''The PowerChrom System can record and analyse data from two detectors on a Waters HPLC.'' | ''The PowerChrom System can record and analyse data from two detectors on a Waters HPLC.'' | ||
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=== Injection Valve === | === Injection Valve === | ||
− | The two black cables from the injection valve are connected to PowerChrom unit’s TRIG + and –, see Figure 4. In the PowerChrom software, the Inject Settings is set to “Wait for Inject”. Turning the injection valve will then trigger the start of the recording. | + | The two black cables from the injection valve are connected to PowerChrom unit’s TRIG + and –, see Figure 4. In the PowerChrom software, the Inject Settings is set to “Wait for Inject”. This setting is shown in Video 1. Turning the injection valve will then trigger the start of the recording. |
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Revision as of 22:59, 10 July 2013
The PowerChrom System can record and analyse data from two detectors on a Waters HPLC.
Contents
Introduction
This application note describes how a Waters HPLC with two detectors and an injection valve, can be connected to either a PowerChrom or e-corder unit. The PowerChrom software is triggered to start recording by the injection valve, and displays the signal from each detector simultaneously. This can be used to upgrade a HPLC which is connected to an integrator.
Waters 430 Conductivity Detector
The conductivity detector is connected to Channel 1 of the PowerChrom unit using a twisted-pair cable with braided shield, as shown in Figure 2. The shield wire should be connected to either the COM connector of the PowerChrom unit, or to a ground connection on the detector, but not to both.
Waters 484 Tunable Absorbance Detector
The absorbance detector is connected to Channel 2 of the PowerChrom unit using a similar cable, shown in Figure 3.
Injection Valve
The two black cables from the injection valve are connected to PowerChrom unit’s TRIG + and –, see Figure 4. In the PowerChrom software, the Inject Settings is set to “Wait for Inject”. This setting is shown in Video 1. Turning the injection valve will then trigger the start of the recording.