Connecting the C4D to the Agilent 1600 CE
This application note describes how to connect the eDAQ C4D system to the Agilent 1600 CE instrument. See this application note for the Agilent 7100 CE instrument.
The eDAQ C4D system can be connected to the Agilent 1600 CE instrument.
- C4D hardware unit, either:
- ET120 C4D Headstage for Capillary Electrophoresis
- Agilent 1600 CE instrument
- Trigger cable:
- For ER815: use the EC027 Trigger cable supplied with the ER815, or
- For ER225: EC071 Agilent 7100 Trigger cable - DB9M to Tinned end
- EC020 Standard Test Solutions for C4D Applications
Installing the C4D Headstage in the Agilent Cartridge
It is easy to mount the ET120 C4D Headstage inside the Agilent CE cartridge. Simply thread the capillary through the channel in the side of the C4D headstage and fix in place.
Ensure that you position the C4D headstage near the end of the capillary that is grounded, away from the end of the capillary where the high voltage is applied. This is to ensure that the high voltage doesn’t arc from the background electrolyte inside the capillary, through the thin wall of the capillary, to the headstage and on to the C4D hardware, which could damage the equipment.
You should ensure that the C4D headstage and its flat grey cable are at least 1 cm away from the capillary as it loops inside the cartridge. This is to prevent the high voltage inside the capillary from shorting through the headstage and its cable, which are both electrically grounded.
You should also make sure that the C4D hardware unit is connected to an electrical socket which is grounded. Connect the C4D hardware directly to the electrical socket in the wall, not using an electrical power board.
Connecting an Agilent Detector to the C4D Hardware Unit
Some Agilent detectors have an analog output. This can be connected to a C4D hardware unit with analog input, such as the ER225 or ER825R, so that signals from both the C4D and Agilent detector can be displayed in the eDAQ PowerChrom software.
Recording the C4D Signal in ChemStation
If you wish to record the C4D signal using the Agilent ChemStation software, the ER815 C4D Detector has an analog output for this. However, the Agilent 1600 CE instrument doesn’t have an analog input, so the Agilent 35900E data acquisition interface is required to accept the signal from the ER815.
- The eDAQ C4D was used with the Agilent CE for the analysis inorganic ions in drinking water. The application note can be viewed here and the research paper at DOI: 10.1080/10826076.2013.825862
- The C4D detected both anions and cations in a single injection. This was done by injecting the sample at both ends of the capillary and placing the C4D headstage at an appropriate position along the capillary.
- It was noted that C4D detection is an alternative and more flexible method of detection compared to the standard optical detection. The effective separation length can easily be adjusted by simply sliding the C4D headstage along the capillary, significantly facilitating the method development and the obtained separation efficiency.
- The estimated limits of detection (0.07 – 2 ppm) and defined limits of quantification (0.3 – 7 ppm) were comparable or better than those described for indirect UV detection, allowing much broader linear ranges (up to 120 ppm) for most of the studied ions.
- The Agilent CE was coupled with the eDAQ C4D for the simultaneous determination of atenolol and amiloride in pharmaceutical preparations. Good analytical performance with regards to linearity, reproducibility, and accuracy was achieved using the CE-C4D method. DOI: 10.1002/bmc.1390
- The determination of cis/trans isomers ofoctadec-9-enoic acid and other long chain fatty acids was performed using the Agilent CE with eDAQ C4D. The method was applied to the determination of fatty acids in margarine samples. The proposed method offers distinct advantages over the GC and HPLC methods, especially in terms of simplicity (without derivatization) and sensitivity. DOI: 10.1016/j.chroma.2013.03.014