Difference between revisions of "Waters HPLC with PowerChrom"
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''The PowerChrom System can record and analyse data from two detectors on a Waters HPLC.'' | ''The PowerChrom System can record and analyse data from two detectors on a Waters HPLC.'' | ||
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| [[File:Figure 2. Waters 430 Conductivity Detector.jpg|x200px|thumb|right|Figure 2. Waters 430 Conductivity Detector]] | | [[File:Figure 2. Waters 430 Conductivity Detector.jpg|x200px|thumb|right|Figure 2. Waters 430 Conductivity Detector]] | ||
+ | | [[File:Figure 3. Waters 484 Tunable Absorbance Detector.jpg|x200px|thumb|right|Figure 3. Waters 484 Tunable Absorbance Detector]] | ||
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| [[File:Figure 4. Waters injection valve.jpg|x200px|thumb|right|Figure 4. Waters injection valve]] | | [[File:Figure 4. Waters injection valve.jpg|x200px|thumb|right|Figure 4. Waters injection valve]] | ||
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Revision as of 22:55, 10 July 2013
The PowerChrom System can record and analyse data from two detectors on a Waters HPLC.
Contents
Introduction
This application note describes how a Waters HPLC with two detectors and an injection valve, can be connected to either a PowerChrom or e-corder unit. The PowerChrom software is triggered to start recording by the injection valve, and displays the signal from each detector simultaneously. This can be used to upgrade a HPLC which is connected to an integrator.
Waters 430 Conductivity Detector
The conductivity detector is connected to Channel 1 of the PowerChrom unit using a twisted-pair cable with braided shield, as shown in Figure 2. The shield wire should be connected to either the COM connector of the PowerChrom unit, or to a ground connection on the detector, but not to both.
Waters 484 Tunable Absorbance Detector
The absorbance detector is connected to Channel 2 of the PowerChrom unit using a similar cable, shown in Figure 3.
Injection Valve
The two black cables from the injection valve are connected to PowerChrom unit’s TRIG + and –, see Figure 4. In the PowerChrom software, the Inject Settings is set to “Wait for Inject”. Turning the injection valve will then trigger the start of the recording.