The eDAQ 150 micron C4D headstage
From eDAQ Wiki
A C4D (capacitively-coupled contactless conductivity detector) headstage is described for use with 150 μm outer diameter capillaries and for the analysis of three cations.
Introduction
eDAQ has developed a C4D headstage for use with capillaries with an outer diameter of 150 μm. The headstage was used for the analysis of a solution containing potassium, sodium and lithium chlorides dissolved in MES/His background electrolyte (BGE). The headstage can be used with any eDAQ C4D hardware.
A limit of detection of 3 µM was obtained.
Experimental
- Capillary from Polymicro Technologies, 150 µm OD, 76 µm ID, 60 cm long.
- Sample: 200 µM Na, K, Li (as chloride salts) dissolved in water and BGE
- BGE: 20 mM of 2-(N-morpholino)ethanesulfonic acid and L-histidine
- The new capillary was prepared by flushing with 0.1 M HCl, water, 0.1 M NaOH, water, then BGE, for 5 minutes each
- Instrument: built in-house at University of Basel
- Injection: 10 s siphoning at 10 cm height
- Separation voltage: 25 kV (10 μA current)
- C4D settings: 700 kHz, 100 V, gain ON
eDAQ Equipment Required
- C4D hardware unit, either:
- ER225 C4D Data System, or
- ER815 C4D Detector with a third-party data acquisition system
- ET120/150 C4D Headstage (150 μm)
