Capillary electrophoresis is a separation method performed by applying a high voltage along a capillary. All ions (positive and negative) are pulled through the capillary in the same direction by the electroosmotic flow. The analytes separate as they travel along the capillary because of differences in their ionic mobility and are detected near the outlet of the capillary.
Contactless conductivity detection can be used for virtually all charged species: inorganic anions and cations, as well as organic ions, such as carboxylic acids, amines, amino acids, peptides, proteins, DNA fragments, antibiotics and many other pharmaceutical compounds. Tagging or other modification of the analytes is usually NOT required, while limits of detection are often comparable to, or sometimes even better, than UV-visible absorption techniques.
The eDAQ C4D can be connected to capillary electrophoresis instruments from Agilent, Beckman Coulter, PrinCE, WynSep or purpose-built instruments.
The C4D system comprises of a hardware unit and headstage. Select one C4D unit (depending on which software you wish to use and how many C4D channels are required) and at least one C4D headstage (depending on which capillary/tubing is being used):
- C4D Data System: Includes PowerChrom software for data collection and analysis.
- C4D Detector: Can be used with third party data acquisition systems.
- C4D multi-channel Detector: Record the conductivity at up to eight positions on a capillary or tubing.
- Capillary Headstage ET120: Connects to capillary with 365 µm outer diameter.
- General Purpose Headstage ET125: For tubing sizes 1/16 inch (1.59 mm) OD or less (specify outer diameter required).
- Standard Test Solutions: Analytes and background electrolyte solutions for testing a C4D system in electrophoresis experiments.